Species boundaries refer to the defining factors that separate one species from another in the natural world. These boundaries can be based on genetic differences, physical characteristics, ecological niche, reproductive isolation, behavior, or any combination of these factors. Understanding species boundaries is important in the fields of taxonomy, ecology, and evolutionary biology, as it helps us classify and understand the diversity of life on Earth. Researchers study species boundaries to determine how closely related different organisms are, how they have evolved over time, and how they interact with each other and their environment. Establishing species boundaries can be a complex and evolving process, especially as new technologies and approaches are developed to study genetic and phenotypic differences between organisms.